The technique usually requires either heat inactivation (2) or partial purification (3-5) of crude protein extract. One of these filters is used to visualize proteins in the lanes by staining, and the other one is for detecting protein factors interacting with a labeled probe (1). The polyacrylamide gel containing the proteins is then sandwiched between two filters so that the proteins can diffuse out and bind onto the filters to produce replicas of the gel. (1) In this original protocol, proteins are first separated based on size by SDS-PAGE. The technique is modified from a previous procedure called 'protein blotting' described by Bowen et al. Southwestern blotting is a technique developed for the detection and characterization of protein factors interacting with distinct DNA motifs. Identification of protein factors that bind to genes to turn them on or off is therefore important in investigating gene functions. Transcription and hence expression regulation of a gene involves initially highly specific interactions of protein factors with distinct short DNA sequence motifs, 6-8 bp in size, usually located within the promoter or other functional cis-acting elements. Southwestern blotting is useful in investigating DNA-protein interactions to aid the identification of DNA-binding protein factors. Understanding the spatial and temporal expression patterns of a gene is essential in elucidating its functions. In this article, a commonly used protocol and expected results are described and discussed. The whole Southwestern blotting procedure takes ~4 d to complete. This technique could be combined with database searching (TransFac, ), prediction of potential protein factors binding onto a target motif (e.g., Patch search), in vitro supershift EMSA and in vivo chromatin immunoprecipitation (ChIP) assays for effective identification of protein factors. The interaction of the probe with the protein(s) is later visualized by autoradiography. This method combines the features of Southern and Western blotting techniques a denaturing SDS-PAGE is first employed to separate proteins electrophoretically based on size, and after transferring the proteins to a membrane support, the membrane-bound proteins are renatured and incubated with a P-labeled double-stranded oligonucleotide probe of specific DNA sequence. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor. Southwestern blotting is used to investigate DNA-protein interactions.
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My personal taxes are on the complicated side, and TurboxTax’s search feature was a huge help. This made the process seamless and ensured all information was entered correctly. I was also able to import some of my 1099-INT and 1099-DIV forms by logging into my respective financial institution websites right from the TurboTax site. If TurboTax doesn’t recognize your employer’s EIN, you can also take a photo of your W-2 form using the TurboTax mobile app. By just entering my employer ID and the dollar amount from box 1, the program was able to automatically import my entire W-2 and populate all the required boxes. With some of the other programs, I had to manually enter each line item from my W-2 form, but not with TurboTax. Out of all four tax programs, entering my W-2 information was by far the easiest with TurboTax. The system will automatically find the TurboTax version you need based on your responses. The site asks a number of questions about common financial items, such as whether or not you have a job, pay rent, pay student loans, sold stock, have children and more. Taxes are complicated, and making sure you get everything accurate while maximizing your deductions is just that: complicated.įrom the moment you arrive at the TurboTax website, the company holds your hand to help you figure out the right software version to use for your specific taxes. Based on our testing, it’s hands down the most customer-friendly option with an easy-to-use platform.īut you may find that you need to pay some of TurboTax’s add-on fees to ensure the most accurate results, meaning you might be better off just handing over your tax documents to an accountant to save the headache. However, if you have more complex taxes - which could include running a small business, having numerous deductions or owning lots of real estate - you’ll want to put cost aside and use TurboTax. Since professional accountant support typically isn’t needed when it comes to a simple tax situation, TaxSlayer should be your go-to for its low-cost option. If you have relatively simple taxes but still more than what’s allowed in the free versions, all of the basic-level paid options will work just fine, so cost might be your biggest factor when selecting one of the four. On the flip side, H&R Block will also work hardest at trying to sell extra options to you throughout the process, so “free” might not end up being truly free if you decide to take them up on one or more of their pitches. Not many forms are included at the most basic level, though H&R Block provides the most forms under this no-cost filing option. However, TaxAct will still charge you to file your state return. The basics of these programs don’t change much from year to year, so if you have a straightforward tax situation - a W-2 from a job and perhaps some interest or dividend income - all of these programs offer a free option that will likely work for you. We revisited the same programs for the current tax year and explored their new features to see if our verdict remains the same.īest for those with W-2 income, dependents, education expenses and retirement incomeīest for freelancers and those with investments or rental property Then, we compared the results to those of a tax professional to see if the programs could get you the same (or better) results. These applications take all your income and expense numbers, calculate your total tax and even electronically file your return for you.īut with many online tax programs available, which one should you use? A few years ago we tested the top four programs by entering our tax information into each program. To make your life less chaotic, you might consider using an online tax program. And while it’s a chore many of us dread, for better or worse, your taxes will be due before you know it. We may receive a commission through our affiliate partners if you apply and are approved for a product, but our reporting is always independent and objective.Īpril 18 - tax day - is a day you won’t want to forget in 2023. Your CNN account Log in to your CNN accountĬNN Underscored reviews financial products based on their overall value. To view the real-time lyrics of a song you’re listening to, go to the Now Playing page and slide up on the Lyrics tab at the bottom of the page. IMAGE: Tarvin Gill / Mashable Southeast Asia And while Genius did provide a backstory to the songs and some other details, it didn’t offer full lyrics.Įventually, Spotify scrambled back to Musixmatch this time around and made sure to roll out the feature to more regions. Spotify had previously worked with Musixmatch to provide the lyric services, but both companies cut ties in 2016. Some countries like Thailand, Vietnam, and Indonesia did have lyrics support, but it was done through a third-party provider. IMAGE: Spotifyīefore this, Spotify did have a lyrics display feature, but it was very much limited to the song and region you’re in. The music streaming company said that the real-time lyric feature was rolled out on June 30, and all you need to do is update the app via the Play Store or App Store for Android and iOS, respectively. From a stability point of view, the tool experienced no lyric mismatching or any other UI-related hiccups during our tests.After years of being selective on which songs will display lyrics, Spotify has announced that it will bring real-time lyrics for all songs to more regions, including Southeast Asia. To conclude, Versefy is a neat and handy application perfect for users interested not only in the musical aspect of a song, but also the core of it - the lyrics. These are Musixmatch,, and, and the settings menu where they're located allows one to enable/disable them at any time. The so-called sources are established web services dedicated to musical knowledge and lyrics collection. The application makes use of three sources from where the lyrics are imported. Furthermore, the text can be visually enhanced by enabling the "Bold Font." A "Focus Mode" can also be activated, which results in removing any UI-related elements, further emphasizing the lyrics. Zooming in the lyrics is also possible, going from an 8px zoom level up to 42. Still, there are some aspects to it that might prove handy in certain situations.įor example, the background can be switched between a dark and white theme. Interface-wise, there is not much to talk about, but that's not necessarily a bad thing, especially when the users are mainly concerned about lyric accuracy. However, Versefy is limited to the desktop version only, so don't try to run this app with the web service, they're not compatible. As long as you are logged in on your account, nothing will be stopping you from using any platform regarding searching for songs and play them. Versefy is a third-party application with a single, well-determined purpose - to instantly display the lyrics of any songs you're listening to on Spotify.Īs many of us are aware, Spotify has both a desktop client as well as a web service. View the lyrics of any song currently playing on Spotify with the help of this lightweight software application that requires no setup It works flawlessly and always recognises the songs that I'm playing.” “Best app! This is the best lyrics app for Spotify hands down! Highly recommended!” Quickly response to the spotify playing.” This app is probably the best among all same ones.” “Perfect! I wish I found this sooner! I love it! Its an app to keep.” This app is one of the must have apps for music lovers.” Does what it's supposed to do, great program.” Personally, this app has truly enhanced my listening experience.” It gives you access to all leading lyrics databases, enabling you to read and sing along your favorite songs. “One of its own kind! This app is undoubtedly the greatest thing that ever happened to music listeners. October’s lunar eclipse will ignite your 12th house of surrender, closure, and unconscious patterns. Something you’ve been creating may finally be coming to light. It’s time to commit to yourself even more deeply than ever before. If your values have shifted, consider this a wake-up call. With Venus-your celestial ruler-transiting dutiful Virgo (via your fifth house of love, passion, and fertility), everything from the topic of children to the pursuit of your artistic craft could be of greater emphasis at this time. How have your relationship dynamics evolved in the past two years? Are your commitments in alignment with your sense of self worth and overall sense of stability? If not, then it may be time to part ways with what is no longer in alignment with you. Read your full monthly horoscope here.Īlthough manifesting and making wishes isn’t advised under the volatile energy of an eclipse, let’s not forget you’re still the main character of this lunar event, Taurus. Take care of yourself like a lover, because there’s a special kind of love that only you can give yourself. Remember who you are and what you’re capable of accomplishing and providing for yourself, without the strings attached to someone else’s help. La luna will also be sitting close to Jupiter retrograde in Taurus, suggesting the likelihood of you being blessed with more abundance and opportunity from now until the end of the year. And while this full-circle moment sheds light on everything you’ve accomplished and manifested over the past year, Venus encourages you to celebrate the little things you find gratitude in the little things. October’s full moon lunar eclipse marks the ending of a major chapter in your life, especially when it comes to your finances, creature comforts, and overall sense of security. Venus will eventually meet with innovative Uranus in a productive trine after the eclipse, bringing forth deeper insights and unveiling game-changing solutions for the future. Do your day-to-day affairs contribute to your sense of security? Do they make you more self-reliant? Is there something that needs to be tweaked or reconsidered? This is an opportunity to integrate a deeper mindfulness when it comes to our craft, thanks to Venus in Virgo. This is highlighting everything from our daily devotions to the way we maximize our routines. Love planet Venus-the celestial ruler of this eclipse-is currently transiting the methodical and practical sign of Virgo. The full moon lunar eclipse in Taurus takes place on October 28 at 4:24 p.m. The Greater Benefic planet is symbolic of expansion, opportunity, and spiritual growth you could experience these new energies before and/or after it stations direct in December. As for the astrology surrounding this eclipse, it is both auspicious and supportive, thanks to the benevolent influence of Jupiter retrograde in Taurus. This marks an emotional turning point, as it will usher in life-altering changes between now and the next six months. A lunar eclipse takes place when the Earth casts its shadow on the moon, making it a more intense and transformative experience. This full moon is not just like any other full moon, but something much stronger and more life-changing. After all, the relationship you have with yourself (Taurus) is a prelude to the relationship you have with others (Scorpio). On the other hand, Scorpio is ruled by Pluto and Mars, symbolizing everything from the way you regenerate after trauma to the way you embrace and accept intimacy. Taurus is ruled by Venus-planet of beauty, security, and self-worth. Where does this astrological axis exist in your birth chart? The astrological houses occupied by these zodiac signs speak to your personal stability and shared resources. This is the final eclipse of 2023, bringing the Taurus-Scorpio eclipse series that started way back in 2021 to a close. What do you need to strengthen, solidify, and commit to in order for you to live your best life? What are you willing to live without in order to receive something even better in the long run? Do not fear-this eclipse is leading you toward your ultimate destiny. In addition to everything you’ve accomplished and realized, this eclipse is highlighting what lacks a practical, consistent, and grounded foundation in your life. How have your commitments and personal values evolved and transformed in the last couple of years? What is no longer fruitful or stable in your life? This month’s full-moon-lunar-eclipse will take place on October 28 at exactly 4:24 p.m. Themes of commitment, financial security, and self-reliance are likely at the top of your mind, as unexpected breakthroughs are encouraging you to move forward in a positive and self-enriching manner. Pay attention to how October’s full moon lunar eclipse will affect each zodiac sign, because it will bring you to the final conclusion of this story. A personal journey you began in 2021 is about to come full-circle. Resolutionator ( $3, direct): Bring back the classic resolution switcher to your menu bar that Apple inexplicably ditched. Simple utilities can make a big difference in how great your Mac is to use, as these beauties prove.Īmphetamine ( £free, Mac App Store): Force your Mac to stay awake indefinitely, temporarily, or based on app/network triggers.īartender ( $15, direct): Bring sanity to your menu bar by organising items and have them appear only when needed.Ĭloud Battery ( £free or £2.49, Mac App Store): Track power levels on all your devices and get alerts when one needs charging. So also backup your Mac to the cloud with Backblaze ($7 per month, direct). Local backups alone are no good when all your kit gets nicked or destroyed in a fire. Superb apps for powering up macOS, to help you work faster, free up space, and ensure you never lose a file. Get OpenEmu (£free, direct) The best Mac essential utilities Use Ctrl-click > Open in Finder and you’ll be on your way to retro bliss. Your Mac will grumble the first time you try to launch OpenEmu. Just watch those chunky pixels don’t take an eye out. There’s also an ‘experimental’ version that adds a load of additional systems, including arcade. If you’ve previously delved into the horrors of emulation, OpenEmu’s ‘it just works’ mentality will appeal. Controllers can be configured, and then you can play. Point it at your games and it’ll download cover art. Fortunately, OpenEmu is one of the best on any platform. The Mac’s not exactly drowning in emulators when you want to get your retro gaming on. And being able to flag books as ‘loaned’ means you should never again lose a precious volume to a forgetful friend.īuy Book Tracker (£free + £9.49, Mac App Store) OpenEmu You can track what you’re currently reading and note down what you thought when you were done. Log your collection in the app by bar-code scans or online searches, and then tag/organise as you see fit. If you often guiltily look at piles of unread books around your home, glance at digital books installed on devices, and then decide to buy yet more books (because, hey, more books), Book Tracker is a must. The app generously gives you all this for free but lob the creator a fiver and you unlock filtering options and can follow record labels.īuy MusicHarbor (£free or £4.99, Mac App Store) Book Tracker Promo videos are available too, like your own personalised MTV. The app then shows latest and upcoming releases, which you can preview or add to your library. You ‘import’ artists from Music or add them manually. Handily, MusicHarbor does that job brilliantly. MusicHarborĪpple’s Music app is fine for playback and surfacing new tunes you’ll like, but it’s rubbish at keeping tabs on favourite artists. When life shifts from work to play, use these apps to entertain yourself. Free Flyer - A very popular and beautifully elegant, vintage attraction in many parks around the world.The ride also utilises the new customisable height system. Golden Eagle - The sister of the Rising Raptor has the suspended swinging cars of its counterpart but also offers a more thrilling outward facing gondola arrangement.This ride can be built from 22 meters to an impressive 58 meters. This ride uses the all new customisable height system which allows players to tailor the ride between easy-going guests in search for impressive views or hardy thrill-seekers in need of a more intense ride experience. Rising Raptor - The Rising Raptor is one of the largest and most complex rides to appear in the game.The ride can be set at 3 spectacular heights as it gracefully ascends and descends. Sky Temple - A unique observation platform ride that’s beautifully themed in a traditional Qin Dynasty architecture.Fixed an issue where the Dive animations on Stunt people were incorrectly named.Improved the texture that Stunt people appear from.Fixed an issue with texture seams on the Stuntman Business animatronics.Fixed an issue with textures on Stunt people.Fixed an issue where some Stunt people animations would clip through their support poles.Fixed an issue where the Movie Camera Crane would continue to animate even if set to Activate On Trigger.Fixed an issue where the Warehouse Door would not revert to its original colours.Fixed an issue where the Tank Explode would not revert to its original colours.Fixed an issue where the Small Truck Box Storage item was lit.Fixed an issue where items would not revert to their original colours if Activate on Trigger was disabled during a fade.Improved the animation for the Fairytale Fall 40m animations.Fixed placement and timing issues with the Alley Scene blueprint.Fixed an issue where animations where stunt people would not offset their animations on placement.Fixed an issue where the game camera could clip below the terrain.General improvements to text for scenery items in the Studios Pack.Fixed an issue where colour channels on the Brownstone Eave Corner Outer were not consistent with the rest of the set.Fixed an issue where the colours for triggerable flexicolour were in the wrong order.Fixed an issue where obtaining a third Hotel perk would rename other perks.
* Sequent time chunks filled with all deep work, or vice versa. * No any loosening-up activities scheduled between blocks. * Too optimistic about the estimated time arranged for a task. * Too strict to the schedule and any changes or flexibility are not allowed. * Keep every minute on the regiment, and make 24 hours fully stuffed. There are mainly five cases that cannot be considered as an appropriate adoption of time blocking. Like what’s being said, even though time blocking is a simple concept from the surface, it can still be misused and taken to extremes. Time Blocking also works for someone who can get a sense of accomplishment and stability from a well-scheduled daily life and who’d wish to continuously look back on his/her performance. In another case, if you’re a fresh graduate who just joined a new company, then maybe the awareness and skills of time management are what you need, to avoid being trapped into the “keeping-busy fallacy” where workload keeps piling up but productivity keeps running low. However, blocking time off has an instant effect on those who have a short-term goal, no matter if it’s an important exam or a job application, because they need to use their time wisely in a short period to maximize output. customer support specialists, whose work is basically based on in-time situations and thus cannot be estimated. Well, the fact is there is never a one-fits-all solution. Time Blocking might be infeasible for reactive workers, e.g. Therefore, time blocking lets you track your time-spending and further reflect on it for higher efficiency. For another, you’ll know the average time spent on certain tasks, which then helps you make realistic plans on time allocation. For one thing, competitive daily/weekly tasks can be quickly arranged. When you get used to scheduling your day into set and controlled units, your own productivity pattern will form. * It helps keep track of your time expenditure With all tasks planned out alongside the day, the anxiety level is also lower because you can easily gain the control power over your workload. With all mental resources invested in a single task, it can avoid getting distracted by miscellanies. * It helps lock your focus and reduce anxietyĭoing one task at a time can lock your focus and keep it intact, compared with splitting your attention across multiple tasks. Combining time blocking and task batching also avoids frequent context-switching and helps minimize your cognitive burden caused by goal-changing. Setting a time frame for shallow work can prevent you from spending too much time on it and save mental spaces for other higher-value tasks. Deep tasks such as coding, designing, and drafting a marketing plan, usually require intensive attention and deep thinking, while shallow tasks are comparatively low-value tasks like emails, team chats and phone calls. There are mainly two types of tasks at work: Deep and shallow. Time blocking is closely linked with task batching, which means similar tasks can be grouped together and distributed to time chunks. * It reduces context switching and mental energy loss How Time Blocking worksįrom the face value, this technique seems just a walk in the park. This comes with higher necessity in today’s society since 24-hour-a-day remains unchanged, while daily information explodes in the modern workplace. Some of the world’s most productive people, from Elon Musk to Bill Gates to Cal Newport (Author of Deep Work) have combined their to-do lists with this important method: Time Blocking.Ī Russian entomologist Alexander Aleksandrovich Lyubishchev, kept recording the expenditure of his time, for 56 years in a row, in order to stay in control of his time. Time Blocking is a time management method that helps us get more done in the finite time by cutting a day into many chunks and each chunk is dedicated to a certain task. Similarly, if we split 24 hours/1440 minutes of a day into blocks, how we spend the time is how we pile up these blocks in order the house to be built. You might instantly recall the Lego blocks that we used to build a house when we were kids. You also have the A1 and A2 cards -also known as Application Performance Class 1 and 2- featuring higher random read and write speeds than non-A1/A2 models, which should lead to faster game loading times since loading a game is more or less a random read operation. UHS-I SD cards come in two flavors regarding minimum read/write speed: UHS Speed Class 1 (U1) models have a minimum read/write performance of 10MBps, while UHS Speed Class 3 (U3) cards have a minimum read/write speed of 30MBps. Specifically, GPD stresses that the Win 4 has a smaller footprint than the Steam Deck, while delivering superior Geekbench 5 benchmark scores and offering more RAM, storage and a larger battery. For instance, Samsung's latest high-end SD card lineup, the Pro Plus, features read rates of up to 180MBps and up to 130MBps write performance. However, you should expect a decent uplift in line with whatever spec bumps that the console might possess. On the other hand, some high-end UHS-I cards feature read and write speeds that go over the Deck's max specs. Steam Deck, the definitive Linux handheld gaming PC to date, has recently crossed the threshold of supporting 12,000 games marked Playable or Verified by Valve. While the Steam Deck wasn’t exactly earth-shattering in comparison to full-fat PC hardware, the Steam Deck 2 will follow suit. On the one hand, you've got older models with read and write speeds of 50MBps or less, notably below the Deck's SD card slot specs. In other words, if you plan on getting an SD card for your Deck, you're limited to UHS-I models. The Steam Deck has an SD card slot that supports UHS-I -also known as UHS-1- SD cards, topping out at about 104MBps for read and write performance. |
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